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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
16/05/2017 |
Data da última atualização: |
22/11/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, J. M. L. dos; MONTEIRO, J. P.; RIBEIRO, W. L. C.; MACEDO, I. T. F.; ARAÚJO FILHO, J. V. de; ANDRE, W. P. P. |
Afiliação: |
Jessica Maria Leite dos Santos, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; JOMAR PATRICIO MONTEIRO, CNPC; Wesley Lyeverton Correia Ribeiro, UECE - Fortaleza, CE, Brazil; Iara Tersia Freitas Macedo, UECE- Fortaleza, CE, Brazil; José Vilemar de Araújo Filho, UECE- Fortaleza, CE, Brazil; Weibson Paz Pinheiro Andre, UECE - Fortaleza, CE, Brazil. |
Título: |
High levels of benzimidazole resistance and beta-tubulin isotype 1 SNP F167Y in Haemonchus contortus populations from Ceará State, Brazil. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Small Ruminant Research, Amsterdam v. 146, p. 48-52, Jan. 2017. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Haemonchus contortus is the most prevalent parasitic nematode in tropical areas, and anthelmintic resistance is a global problem. Our objective was to characterize benzimidazole (BZ) resistance in gastrointestinal nematode populations in Ceará State, Brazil, using the egg hatch test (EHT) and in H. contortus populations using quantitative real-time polymerase chain reaction (qPCR). Twenty locations were surveyed, and fecal samples were collected from a minimum of 40 animals from each farm and pooled. Five thousand L3 from each farm were used to infect single animals at Embrapa (Brazilian Agricultural Research Company) to provide a source of eggs for both phenotypical and molecular tests. The mean EHT was 2.46 ug/ml (±0.58 g/ml), and BZ resistance was detected at all surveyed locations. The mean resistant allelic frequencies at positions F200Y and F167Y were 34.16% (±12.13%) and 58.31% (±18.89%), respectively. The resistant allelic frequencies at F167Y were higher than those at F200Y in most studied locations. We also investigated the possibility that specific BZ utilization may influence resistant allelic frequencies. We selected three nematode populations based on the resistant SNP prevalence at F200Y and F167Y as follows: higher frequency at SNP F200Y, higher frequency at SNP F167Y and similar frequencies at both positions. Anthelmintic treatments included two BZs (oxfendazole and albendazole) and ivermectin. Three animals per population per treatment were infected with 5000 L3, and nematode eggs were collected for molecular test before and after anthelmintic treatments. The results showed preferential selection of SNP F167Y in response to oxfendazole, an increase in resistant SNP frequencies in general in response to albendazole and little change in relation to pre-treatment situations in response to ivermectin. Our results confirm that BZ resistance is common. The resistant allele at SNP F167Y in H. contortus prevails in Ceará State, and we provide evidence that this result may be due to the utilization of oxfendazole in recent years. MenosAbstract: Haemonchus contortus is the most prevalent parasitic nematode in tropical areas, and anthelmintic resistance is a global problem. Our objective was to characterize benzimidazole (BZ) resistance in gastrointestinal nematode populations in Ceará State, Brazil, using the egg hatch test (EHT) and in H. contortus populations using quantitative real-time polymerase chain reaction (qPCR). Twenty locations were surveyed, and fecal samples were collected from a minimum of 40 animals from each farm and pooled. Five thousand L3 from each farm were used to infect single animals at Embrapa (Brazilian Agricultural Research Company) to provide a source of eggs for both phenotypical and molecular tests. The mean EHT was 2.46 ug/ml (±0.58 g/ml), and BZ resistance was detected at all surveyed locations. The mean resistant allelic frequencies at positions F200Y and F167Y were 34.16% (±12.13%) and 58.31% (±18.89%), respectively. The resistant allelic frequencies at F167Y were higher than those at F200Y in most studied locations. We also investigated the possibility that specific BZ utilization may influence resistant allelic frequencies. We selected three nematode populations based on the resistant SNP prevalence at F200Y and F167Y as follows: higher frequency at SNP F200Y, higher frequency at SNP F167Y and similar frequencies at both positions. Anthelmintic treatments included two BZs (oxfendazole and albendazole) and ivermectin. Three animals per population per treatment were infect... Mostrar Tudo |
Palavras-Chave: |
B-tubulin; Brasil; Ceará; Nematóide gastrointestinal; Real-time PCR; Resistance to anthelmintics; SNP. |
Thesagro: |
Anti-helmíntico; Benzimidazol; Haemonchus Contortus; Helminto gastrintestinal; Ovino; Resistência. |
Thesaurus Nal: |
Benzimidazoles; Brazil; Nematoda; Sheep. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 03219naa a2200385 a 4500 001 2069622 005 2018-11-22 008 2017 bl uuuu u00u1 u #d 100 1 $aSANTOS, J. M. L. dos 245 $aHigh levels of benzimidazole resistance and beta-tubulin isotype 1 SNP F167Y in Haemonchus contortus populations from Ceará State, Brazil.$h[electronic resource] 260 $c2017 520 $aAbstract: Haemonchus contortus is the most prevalent parasitic nematode in tropical areas, and anthelmintic resistance is a global problem. Our objective was to characterize benzimidazole (BZ) resistance in gastrointestinal nematode populations in Ceará State, Brazil, using the egg hatch test (EHT) and in H. contortus populations using quantitative real-time polymerase chain reaction (qPCR). Twenty locations were surveyed, and fecal samples were collected from a minimum of 40 animals from each farm and pooled. Five thousand L3 from each farm were used to infect single animals at Embrapa (Brazilian Agricultural Research Company) to provide a source of eggs for both phenotypical and molecular tests. The mean EHT was 2.46 ug/ml (±0.58 g/ml), and BZ resistance was detected at all surveyed locations. The mean resistant allelic frequencies at positions F200Y and F167Y were 34.16% (±12.13%) and 58.31% (±18.89%), respectively. The resistant allelic frequencies at F167Y were higher than those at F200Y in most studied locations. We also investigated the possibility that specific BZ utilization may influence resistant allelic frequencies. We selected three nematode populations based on the resistant SNP prevalence at F200Y and F167Y as follows: higher frequency at SNP F200Y, higher frequency at SNP F167Y and similar frequencies at both positions. Anthelmintic treatments included two BZs (oxfendazole and albendazole) and ivermectin. Three animals per population per treatment were infected with 5000 L3, and nematode eggs were collected for molecular test before and after anthelmintic treatments. The results showed preferential selection of SNP F167Y in response to oxfendazole, an increase in resistant SNP frequencies in general in response to albendazole and little change in relation to pre-treatment situations in response to ivermectin. Our results confirm that BZ resistance is common. The resistant allele at SNP F167Y in H. contortus prevails in Ceará State, and we provide evidence that this result may be due to the utilization of oxfendazole in recent years. 650 $aBenzimidazoles 650 $aBrazil 650 $aNematoda 650 $aSheep 650 $aAnti-helmíntico 650 $aBenzimidazol 650 $aHaemonchus Contortus 650 $aHelminto gastrintestinal 650 $aOvino 650 $aResistência 653 $aB-tubulin 653 $aBrasil 653 $aCeará 653 $aNematóide gastrointestinal 653 $aReal-time PCR 653 $aResistance to anthelmintics 653 $aSNP 700 1 $aMONTEIRO, J. P. 700 1 $aRIBEIRO, W. L. C. 700 1 $aMACEDO, I. T. F. 700 1 $aARAÚJO FILHO, J. V. de 700 1 $aANDRE, W. P. P. 773 $tSmall Ruminant Research, Amsterdam$gv. 146, p. 48-52, Jan. 2017.
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Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
10/12/2014 |
Data da última atualização: |
10/12/2014 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
TEIXEIRA, W. |
Afiliação: |
WENCESLAU GERALDES TEIXEIRA, CNPS. |
Título: |
How to predict soil water holding capacity using physically based equations? |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: WORKSHOP ON POROUS MEDIA, 1., 2014, Rio de Janeiro. Program & abstracts. Rio de Janeiro: Instituto Nacional de Matemática Pura e Aplicada, 2014. p. 17-18. |
Idioma: |
Inglês |
Conteúdo: |
The soil surface and porosity govern the capacity of soil to hold water. In soil science, many functions were developed to estimate parameters of equations to describe soil water retention and soil hydraulic properties. These functions were created using statistical techniques based in soil properties parameters less laborious and cheaply obtained than the direct measurements of soil hydraulic parameters. These functions are called pedotransfer functions (PTF) and fill the gap between the available soil data and the properties required for modeling soil water fluxes. The physically based equations approach to obtain functions of water retention and hydraulic conductivity of the porous media (soils) may be advantageous, as the predictors will not be select by statistical procedures and be strongly influenced by the available data base and technique used, moreover the predictors will have also a physical meaning. Examples of physically based equations to predict soil water content in function of dielectric conductivity in soils is given and the demand of physically based equation to predict soil water hold capacity and soil hydraulic conductivity is addressed. |
Palavras-Chave: |
Capacidade de retenção de água no solo; Equação de base física. |
Thesagro: |
Retenção de água no solo. |
Thesaurus NAL: |
Soil water retention. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/113440/1/Interpore-p.17-18-2014.pdf
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Marc: |
LEADER 01823nam a2200157 a 4500 001 2002101 005 2014-12-10 008 2014 bl uuuu u00u1 u #d 100 1 $aTEIXEIRA, W. 245 $aHow to predict soil water holding capacity using physically based equations?$h[electronic resource] 260 $aIn: WORKSHOP ON POROUS MEDIA, 1., 2014, Rio de Janeiro. Program & abstracts. Rio de Janeiro: Instituto Nacional de Matemática Pura e Aplicada, 2014. p. 17-18.$c2014 520 $aThe soil surface and porosity govern the capacity of soil to hold water. In soil science, many functions were developed to estimate parameters of equations to describe soil water retention and soil hydraulic properties. These functions were created using statistical techniques based in soil properties parameters less laborious and cheaply obtained than the direct measurements of soil hydraulic parameters. These functions are called pedotransfer functions (PTF) and fill the gap between the available soil data and the properties required for modeling soil water fluxes. The physically based equations approach to obtain functions of water retention and hydraulic conductivity of the porous media (soils) may be advantageous, as the predictors will not be select by statistical procedures and be strongly influenced by the available data base and technique used, moreover the predictors will have also a physical meaning. Examples of physically based equations to predict soil water content in function of dielectric conductivity in soils is given and the demand of physically based equation to predict soil water hold capacity and soil hydraulic conductivity is addressed. 650 $aSoil water retention 650 $aRetenção de água no solo 653 $aCapacidade de retenção de água no solo 653 $aEquação de base física
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